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bbsi digested pdcc6 vector  (New England Biolabs)


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  • 99

    Structured Review

    New England Biolabs bbsi digested pdcc6 vector
    Bbsi Digested Pdcc6 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bbsi+digested+pdcc6+vector/T4+DNA+Ligase/pmc06114144-422-41-49
    Average 99 stars, based on 33541 article reviews
    bbsi digested pdcc6 vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    CRISPR:

    Article Title: The hpRNA/RNAi pathway is essential to resolve intragenomic conflict in the Drosophila male germline
    Article Snippet: .. CRISPR/Cas9 Mutagenesis of D. simulans sgRNAs were designed using http://flycrispr.molbio.wisc.edu/ , and were designed to delete most of the target gene and replace it with a 3xP3:DsRed marker. sgRNA oligo pairs were phosphorylated by T4 PNK (NEB), annealed and ligated into BbsI digested pDCC6 vector using T4 DNA ligase (NEB). ..

    Mutagenesis:

    Article Title: The hpRNA/RNAi pathway is essential to resolve intragenomic conflict in the Drosophila male germline
    Article Snippet: .. CRISPR/Cas9 Mutagenesis of D. simulans sgRNAs were designed using http://flycrispr.molbio.wisc.edu/ , and were designed to delete most of the target gene and replace it with a 3xP3:DsRed marker. sgRNA oligo pairs were phosphorylated by T4 PNK (NEB), annealed and ligated into BbsI digested pDCC6 vector using T4 DNA ligase (NEB). ..

    Marker:

    Article Title: The hpRNA/RNAi pathway is essential to resolve intragenomic conflict in the Drosophila male germline
    Article Snippet: .. CRISPR/Cas9 Mutagenesis of D. simulans sgRNAs were designed using http://flycrispr.molbio.wisc.edu/ , and were designed to delete most of the target gene and replace it with a 3xP3:DsRed marker. sgRNA oligo pairs were phosphorylated by T4 PNK (NEB), annealed and ligated into BbsI digested pDCC6 vector using T4 DNA ligase (NEB). ..

    Article Title: The hpRNA/RNAi pathway is essential to resolve intragenomic conflict in the Drosophila male germline
    Article Snippet: .. sgRNAs were designed using http://flycrispr.molbio.wisc.edu/ , and were designed to delete most of the target gene and replace it with a 3xP3:DsRed marker. sgRNA oligo pairs were phosphorylated by T4 PNK (NEB), annealed and ligated into BbsI digested pDCC6 vector using T4 DNA ligase (NEB). ..



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